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nitrocellulose nc membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad nitrocellulose nc membrane
    Nitrocellulose Nc Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 32538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocellulose+nc+membrane/Nitrocellulose+Membrane/pm42045911-173-14-18
    Average 99 stars, based on 32538 article reviews
    nitrocellulose nc membrane - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Membrane:

    Article Title: Overexpression of the erythropoietin receptor is associated with upregulation of tubulin beta 6 and paclitaxel resistance in ovarian adenocarcinoma cells.
    Article Snippet: Protein concentration was determined using a detergent-compatible protein assay (Bio-Rad Laboratories, Hercules, CA, USA). .. Equal amounts of protein (30 μg), supplemented with 0.01% bromophenol blue, 1% 2-mercaptoethanol, 0.4% SDS, and 5% glycerol, were separated on a 10% SDS-polyacrylamide gel (acrylamide/bis solution, 37.5:1) and transferred onto a nitrocellulose (NC) membrane (Bio-Rad Laboratories) via dry transfer for 10 minutes using the iBlot 2 Gel Transfer Device (ThermoFisher, USA). ..

    Article Title: Foot-and-mouth disease virus-like particle mRNA vaccine induces strong humoral and cellular immunity
    Article Snippet: .. Subsequently, proteins were transferred onto a nitrocellulose (NC) membrane using a Bio-Rad semi-dry transfer apparatus and blocked with 5% skim milk for 1 h. The membrane was then washed three times with PBST and incubated with a pig anti-FMDV polyclonal antibody (dilution ratio 1:1000) at room temperature for 1 h. After another three washes with PBST, the membrane was incubated with an HRP-labeled goat anti-pig secondary antibody (Bioss, bs-0309Rs) at room temperature for 1 h. Finally, the membrane was washed three times with PBST, and the protein bands were visualized using an ECL chemiluminescence system (CLINX, China). ..

    Article Title: Foot-and-mouth disease virus-like particle mRNA vaccine induces strong humoral and cellular immunity.
    Article Snippet: .. Subsequently, proteins were transferred onto a nitrocellulose (NC) membrane using a Bio-Rad semi-dry transfer apparatus and blocked with 5% skim milk for 1 h. The membrane was then washed three times with PBST and incubated with a pig antiFMDV polyclonal antibody (dilution ratio 1:1000) at room temperature for 1 h. After another three washes with PBST, the membrane was incubated with an HRP-labeled goat antipig secondary antibody (Bioss, bs-0309Rs) at room temperature for 1 h. Finally, the membrane was washed three times with PBST, and the protein bands were visualized using an ECL chemiluminescence system (CLINX, China). ..

    Article Title: Targeting Leishmania donovani Sphingosine Kinase 1 using PF-543 enhances immune response and limits parasite load.
    Article Snippet: .. Proteins were then transferred onto nitrocellulose (NC) membrane using an electrophoretic transfer cell (Bio-Rad Laboratories, USA) at RT. ..

    Article Title: Symbiotic exclusivity between CLOCK and TFPI2 drives stemness and immunosuppression in glioblastoma models
    Article Snippet: The cell lysate was mixed with 4x Laemmli sample buffer and then boiled at 95°C for 10 min. Then the boiled protein sample was added to SurePAGE gels (GenScript, #M00653). .. The gels were run at a constant voltage of 60 V and then transferred to 0.2 m nitrocellulose (NC) membrane (Bio-Rad, #1620112) using standard protocol for 30 min in the Trans-Blot Turbo system (Bio-Rad). ..

    Article Title: Targeting Leishmania donovani Sphingosine Kinase 1 using PF-543 enhances immune response and limits parasite load
    Article Snippet: .. Proteins were then transferred onto nitrocellulose (NC) membrane using an electrophoretic transfer cell (Bio-Rad Laboratories, USA) at RT. ..

    Article Title: Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans
    Article Snippet: .. 30 μg proteins were loaded in 10% SDS-PAGE for protein electrophoresis and transferred to nitrocellulose (NC) membrane by Bio-Rad system. ..

    Article Title: Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans.
    Article Snippet: .. 30 μg proteins were loaded in 10% SDS-PAGE for protein electrophoresis and transferred to nitrocellulose (NC) membrane by Bio-Rad system. ..

    Incubation:

    Article Title: Foot-and-mouth disease virus-like particle mRNA vaccine induces strong humoral and cellular immunity
    Article Snippet: .. Subsequently, proteins were transferred onto a nitrocellulose (NC) membrane using a Bio-Rad semi-dry transfer apparatus and blocked with 5% skim milk for 1 h. The membrane was then washed three times with PBST and incubated with a pig anti-FMDV polyclonal antibody (dilution ratio 1:1000) at room temperature for 1 h. After another three washes with PBST, the membrane was incubated with an HRP-labeled goat anti-pig secondary antibody (Bioss, bs-0309Rs) at room temperature for 1 h. Finally, the membrane was washed three times with PBST, and the protein bands were visualized using an ECL chemiluminescence system (CLINX, China). ..

    Article Title: Foot-and-mouth disease virus-like particle mRNA vaccine induces strong humoral and cellular immunity.
    Article Snippet: .. Subsequently, proteins were transferred onto a nitrocellulose (NC) membrane using a Bio-Rad semi-dry transfer apparatus and blocked with 5% skim milk for 1 h. The membrane was then washed three times with PBST and incubated with a pig antiFMDV polyclonal antibody (dilution ratio 1:1000) at room temperature for 1 h. After another three washes with PBST, the membrane was incubated with an HRP-labeled goat antipig secondary antibody (Bioss, bs-0309Rs) at room temperature for 1 h. Finally, the membrane was washed three times with PBST, and the protein bands were visualized using an ECL chemiluminescence system (CLINX, China). ..

    SDS Page:

    Article Title: Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans
    Article Snippet: .. 30 μg proteins were loaded in 10% SDS-PAGE for protein electrophoresis and transferred to nitrocellulose (NC) membrane by Bio-Rad system. ..

    Article Title: Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans.
    Article Snippet: .. 30 μg proteins were loaded in 10% SDS-PAGE for protein electrophoresis and transferred to nitrocellulose (NC) membrane by Bio-Rad system. ..

    Protein Electrophoresis:

    Article Title: Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans
    Article Snippet: .. 30 μg proteins were loaded in 10% SDS-PAGE for protein electrophoresis and transferred to nitrocellulose (NC) membrane by Bio-Rad system. ..

    Article Title: Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegans.
    Article Snippet: .. 30 μg proteins were loaded in 10% SDS-PAGE for protein electrophoresis and transferred to nitrocellulose (NC) membrane by Bio-Rad system. ..



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